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330 CMR:   DEPARTMENT OF FOOD AND AGRICULTURE
9/20/96
330 CMR - 162
27.13:   continued
(3)   Screening and confirmatory methods for the detection of abnormal milk.
(4)   Any other tests may be required which have been approved by the U.S. Food and Drug
Administration and the Commissioner to be equally accurate, precise, and practical.
(5)   Any one of the following three tests may be used for screening raw milk samples to
indicate a range of somatic cell levels: California Mastitis Test, Modified Whitside Test, or
Wisconsin Mastitis Test.
(6)   One of the following confirmatory tests shall be used:  Direct Microscopic Somatic Cell
Counting, Electronic Somatic Cell Counting, Optical Somatic Cell Counting for Membrane
Filter DNA Somatic Cell Count, or any other standard procedure previously approved, in
writing, by the Department.
(7) Laboratories using acceptable screening tests shall confirm that sample of herd milk
which exceeds any of the following screening test results:
(a)   California Mastitis Test--1
(b)   Modified Whitside Test--1+
(c)   Wisconsin Mastitis Test--18 mm
The results of the screening test, or confirmatory test, shall be recorded on the official
records of the dairy farm and a copy of the results sent to the milk producer.
(8)   When a warning letter has been sent because of excessively high somatic cell counts, an
official inspection of the dairy shall be made by regulatory personnel. This inspection shall be
made during milking time.
(9)   When bacterial counts and temperature determinations are made of several samples of
the same milk collected from the same supply on the same day these values are averaged
arithmetically, and the results recorded as the count for that day.
(10)   A computer or other information retrieval system may be used.
(E)Disease Transmission
(1)   When samples of raw milk are taken, they shall be drawn following adequate agitation.
(2)   Raw milk must be produced under clean, sanitary conditions to limit the possibility of
any disease transmission via raw milk.
(3)   Raw milk is known to transmit disease, including (but not limited to):
(a)   Listeriosis;
(b)   Yersiniosis;
(c)   Campylobacteriosis;
(d)   Salmonellosis;
(e)   Brucellosis;
(f)   Q-fever;
(g)   Tuberculosis; and
(h)   Any other disease so designated by the Department.
27.14:   Enforcement Standards
(A)Enforcement
(1)   Whenever two of the last four consecutive bacterial counts, somatic cell counts, or
cooling temperature checks, taken on separate days, exceed the limit of the standard for the
milk and/or milk products, the Department shall send by first class mail a written notice
thereof to the certificatee. This notice shall also inform the certificatee of the provisions of
330 CMR 27.14(A)(2), and shall remain in effect as long as two of the last four consecutive
samples exceed the limit of the standard. An additional sample shall be taken within 21 days
of the sending of such notice, but not before the lapse of three  days.
(2)   Whenever the standard is violated by three of the last five bacterial counts, somatic cell
counts, or cooling temperature checks, the Department shall immediately suspend the
certificate in accordance with 330 CMR 27.16(A).
(3)   The department may accept determinations of 330 CMR 27.14 by out-of-state regulatory
authorities for producers shipping milk into the Commonwealth from out-of-state locations.
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